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elisa assays  (Boster Bio)


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    Structured Review

    Boster Bio elisa assays
    Elisa Assays, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+elisa+kit/Human+IFN-Beta+ELISA+Kit+PicoKine/pm42826488-87-20-29
    Average 93 stars, based on 5 article reviews
    elisa assays - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Periostin drives extracellular matrix degradation, stemness, and chemoresistance by activating the MAPK/ERK signaling pathway in triple-negative breast cancer cells.
    Article Snippet: The following primary antibodies were used: anti–periostin (Proteintech, #66,491–1–lg, 1:3000), anti–ERK1/2 (CST, #9102, 1:1000), anti–phospho ERK1/2 (CST, #4370, 1:1000), anti–P38 (CST, #9212, 1:1000), anti–phospho P38 (CST, #9215, 1:1000), and anti–β–actin (Affinity Biosciences, #AF7018, 1:1000). .. The conditioned medium (CM) of TNBCs cultured under different conditions was collected, and the concentration of periostin was determined with a human ELISA kit (Boster, California, USA) according to the manufacturer’s protocols. .. To determine the proliferation of cells under different culture conditions, cell proliferation assays were performed using a CCK–8 kit (Beyotime, Shanghai, China) according to the manufacturer’s instructions.

    Concentration Assay:

    Article Title: Periostin drives extracellular matrix degradation, stemness, and chemoresistance by activating the MAPK/ERK signaling pathway in triple-negative breast cancer cells.
    Article Snippet: The following primary antibodies were used: anti–periostin (Proteintech, #66,491–1–lg, 1:3000), anti–ERK1/2 (CST, #9102, 1:1000), anti–phospho ERK1/2 (CST, #4370, 1:1000), anti–P38 (CST, #9212, 1:1000), anti–phospho P38 (CST, #9215, 1:1000), and anti–β–actin (Affinity Biosciences, #AF7018, 1:1000). .. The conditioned medium (CM) of TNBCs cultured under different conditions was collected, and the concentration of periostin was determined with a human ELISA kit (Boster, California, USA) according to the manufacturer’s protocols. .. To determine the proliferation of cells under different culture conditions, cell proliferation assays were performed using a CCK–8 kit (Beyotime, Shanghai, China) according to the manufacturer’s instructions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Periostin drives extracellular matrix degradation, stemness, and chemoresistance by activating the MAPK/ERK signaling pathway in triple-negative breast cancer cells.
    Article Snippet: The following primary antibodies were used: anti–periostin (Proteintech, #66,491–1–lg, 1:3000), anti–ERK1/2 (CST, #9102, 1:1000), anti–phospho ERK1/2 (CST, #4370, 1:1000), anti–P38 (CST, #9212, 1:1000), anti–phospho P38 (CST, #9215, 1:1000), and anti–β–actin (Affinity Biosciences, #AF7018, 1:1000). .. The conditioned medium (CM) of TNBCs cultured under different conditions was collected, and the concentration of periostin was determined with a human ELISA kit (Boster, California, USA) according to the manufacturer’s protocols. .. To determine the proliferation of cells under different culture conditions, cell proliferation assays were performed using a CCK–8 kit (Beyotime, Shanghai, China) according to the manufacturer’s instructions.

    Article Title: Clinical Study on the Implications of Immunological Markers in the Diagnosis of Periodontitis in People with Diabetes Mellitus
    Article Snippet: .. We used the human ELISA kit from Boster Biological Technology Co., Ltd. U.S.A. for the detection of IL-1β, IL-4, IL-8, and TNF-α. ..

    Article Title: Bufalin targets the SRC-3/c-Myc pathway in chemoresistant cells to regulate metastasis induced by chemoresistance in colorectal cancer
    Article Snippet: .. CXCL16, PDGFAA, VEGFA, IL-6 and ANG levels were measured with a Human ELISA Kit (BOSTER, China) following the manufacturer’s instructions. .. GraphPad Prism 6.02 software was applied to conduct statistical analysis on relevant experimental data.

    Article Title: Lactose-Modified Hyaluronic Acid Molecule Attenuates In Vitro Chondrocyte Inflammation
    Article Snippet: TNF-α and IL-1β levels were measured using ELISA kits from R&D Systems (Minneapolis, MN, USA). .. IL-6 was quantified using a human ELISA kit from Boster Bio (Pleasanton, TX, USA), and Galectin-3 (Gal-3) was measured using an ELISA kit from Sino Biological (Wayne, PA, USA). ..

    Article Title: IL-3 Modulates Microglia Polarization and Attenuates Neuroinflammation in Traumatic Brain Injury.
    Article Snippet: .3 Enzyme-Linked Immunosorbent Assay ELISA) S and CSF from humans and rats, as well as the supernatants rom C8D1A, primary microglia, and BV2 derived from mice, ere collected and detected using ELISA kits. .. The analysis as performed according to the manufacturer’s instructions. ncluding IL-3 human ELISA kit (EK0402, BOSTER, Wuhan, hina), IL-3 rat ELISA kit (EK1324, BOSTER, Wuhan, China), L-6 rat-specific ELISA kit (EK0412, BOSTER, Wuhan, China), NF- α rat-specific ELISA kit (EK0526, BOSTER, Wuhan, China), F images showing that the expression levels of P65 in microglia with or witho mages showing the expression levels of p-P65 and I κB α in microglia with or w he mean ± SD. ns, not statistically significant. ..

    Article Title: Bufalin targets the SRC-3/c-Myc pathway in chemoresistant cells to regulate metastasis induced by chemoresistance in colorectal cancer.
    Article Snippet: .. CXCL16, PDGFAA, VEGFA, IL-6 and ANG levels were measured with a Human ELISA Kit (BOSTER, China) following the manufacturer’s instructions. .. GraphPad Prism 6.02 software was applied to conduct statistical analysis on relevant experimental data.

    Article Title: Lactose-Modified Hyaluronic Acid Molecule Attenuates In Vitro Chondrocyte Inflammation.
    Article Snippet: TNF-α and IL-1β levels were measured using ELISA kits from R&D Systems (Minneapolis, MN, USA). .. IL-6 was quantified using a human ELISA kit from Boster Bio (Pleasanton, TX, USA), and Galectin-3 (Gal-3) was measured using an ELISA kit from Sino Biological (Wayne, PA, USA). ..

    Expressing:

    Article Title: IL-3 Modulates Microglia Polarization and Attenuates Neuroinflammation in Traumatic Brain Injury.
    Article Snippet: .3 Enzyme-Linked Immunosorbent Assay ELISA) S and CSF from humans and rats, as well as the supernatants rom C8D1A, primary microglia, and BV2 derived from mice, ere collected and detected using ELISA kits. .. The analysis as performed according to the manufacturer’s instructions. ncluding IL-3 human ELISA kit (EK0402, BOSTER, Wuhan, hina), IL-3 rat ELISA kit (EK1324, BOSTER, Wuhan, China), L-6 rat-specific ELISA kit (EK0412, BOSTER, Wuhan, China), NF- α rat-specific ELISA kit (EK0526, BOSTER, Wuhan, China), F images showing that the expression levels of P65 in microglia with or witho mages showing the expression levels of p-P65 and I κB α in microglia with or w he mean ± SD. ns, not statistically significant. ..



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    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by <t>DuoSet‐ELISA</t> kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.
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    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by <t>DuoSet‐ELISA</t> kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.
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    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by <t>DuoSet‐ELISA</t> kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.
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    Image Search Results


    Journal: Advances in Pharmacological and Pharmaceutical Sciences

    Article Title: Evaluation of Biological Effect of Triticum vulgare Extract Compared With Hyaluronic Acid in In Vitro Tissue Models

    doi: 10.1155/adpp/8857215

    Figure Lengend Snippet: Primer sequences used for qRT‐PCR.

    Article Snippet: Supernatants of scratched HaCaT cells (treated and untreated) and supernatants of 3D skin previously treated with LPS and after being scratched were collected after 48 and 72 h, respectively, and analyzed using ELISA for quantification of HBD2 production (Elabscience) according to the manufacturer’s instructions.

    Techniques:

    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by DuoSet‐ELISA kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.

    Journal: Chembiochem

    Article Title: Functional Heterogeneity of the Autism Spectrum Disorder‐Associated Gut Microbial Ecosystem Revealed by Fecal Lipopolysaccharides and Bacterial Proteomic Profiling

    doi: 10.1002/cbic.70557

    Figure Lengend Snippet: Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by DuoSet‐ELISA kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.

    Article Snippet: Cytokine levels (pg/mL) in HMC3 supernatants were measured using DuoSet ELISA kits (R&D Systems, Minneapolis, MN, USA) for human IL‐8 (#DY208) and IL‐6 detection (#DY206).

    Techniques: Activation Assay, MTT Assay, Negative Control, Enzyme-linked Immunosorbent Assay, Comparison